Dietary flavonoid fisetin induces a forced exit from mitosis by targeting the mitotic spindle checkpoint

Dietary flavonoid fisetin induces a forced exit from mitosis by targeting the mitotic spindle checkpoint.

Fisetin is a natural flavonol present in edible vegetables, fruits and wine at 2-160 microg/g concentrations and an ingredient in nutritional supplements with much higher concentrations. The compound has been reported to exert anticarcinogenic effects as well as antioxidant and anti-inflammatory activity via its ability to act as an inhibitor of cell proliferation and free radical scavenger, re...

The mitotic spindle checkpoint

One of the most awe-inspiring demonstrations in biology is a timelapse video of mitosis viewed with high magnification phase contrast or Nomarski DIC microscopy. From initial chaotic movement comes the orderly alignment of chromosomes at the metaphase plate followed by a flawlessly synchronous splitting of the chromosomes and their gliding separation to the two mitotic poles. Enforcing the timi...

Exit from Mitosis Spindle Pole Power

of mitosis when the bud cell body receives a progeny spindle pole. The signal that this has occurred is generated when a GTP binding protein located on the outside of the spindle pole is brought into the vicinity of an activator confined to the bud. Thus, the outcome of a motile process, mitotic spindle-mediated segregation of replicated chromosomes, is monitored spatially. To the observer, one...

The cortical protein Lte1 promotes mitotic exit by inhibiting the spindle position checkpoint kinase Kin4

The spindle position checkpoint (SPOC) is an essential surveillance mechanism that allows mitotic exit only when the spindle is correctly oriented along the cell axis. Key SPOC components are the kinase Kin4 and the Bub2-Bfa1 GAP complex that inhibit the mitotic exit-promoting GTPase Tem1. During an unperturbed cell cycle, Kin4 associates with the mother spindle pole body (mSPB), whereas Bub2-B...

Lte 1 promotes mitotic exit by controlling the localization of the spindle position checkpoint kinase